SERVICE

SG ID™ (SG ID™: Drug target ID or Repositioning service)

Concept (Introduction)

 

We have developed a novel method called the SG Cap™ Drug Target Identification (SG ID™), which utilizes a drug or chemical embedded sol-gel microarray coupled with LC-MS/MS. SG ID™ is total solution service for users interested in studying a particular molecule. At the user's request, PCL will immobilize and identify the desired material (e.g. drug, chemical, protein, etc) for new drug screening and development.

 

•  SG Cap™ technology can encapsulate desired material without modifying it in any way, thus ensuring that its active

    sites remain free to bind target molecule. The desired molecule will also be encapsulated at higher concentrations,

    further ensuring a high level of binding activity.

•  The SG ID™ service makes it possible to understand pharmacological mechanisms by examining the binding

    partners of new candidate drugs.

      - Suitable for High - Throughput Technology.

      - Enables users to search for new candidate drugs (or drugs yet to be developed) for various disease by using an FDA approved compound library

      - Provide an opportunity to rediscover the high value of low risk compounds

•  Provides a way to examine molecular interactions not easily done with existing research methods.

•  Can be used to confirm Drug-protein interactions and to screen for interaction partners

      - Collect target material for a fixed candidate drug through affinity interaction

      - Identify the target protein by using mass spectrometry (MS).

     Applicable fields

      - Protein-Chemical interaction (e.g. drug)

      - Antibody-Antigen interaction

      - Enzyme-Substrate interaction

      - Protein-Lipid interaction

      - Protein-Nucleic acid interaction

 

     Examples of molecular interactions revealed by using SG ID™

Example I : for drug repositioning

Example II : Identification of target protein I (Chemical Compound A: D. University, Korea)

Example III : Identification of target protein II (chemical Compound B: C company, USA)

References

1. J.Ahn, et al., A Sol-gel based Microfluidics System Enhances the Efficiency of RNA Aptamer Selection,Oligo nucleotides, 2011, 21(2), 93-100

2. J.Ahn, et al., Aptamer Microarray Mediated Capture and Mass Spectrometry Identification of omarker in Serum Samples,Journal of Proteome Research, 2010, 9, 5568-5573

3. J.Ahn, et al., Selection and Elution of Aptamers Using Nanoporous Sol-gel Arrays with Integrated Microheaters.Lab-on-a-Chip, 2009, 9(9), 1206-1212

4. J.Kwon, et al., High Diagnostic Accuracy of Antigen Microarray for Sensitive Detection of Hepatitis C Virus Infection.Clinical Chemistry, 2008, 54(2), 424-428